Measuring kLa For Better Bioreactor Performance
Knowledge of kLa, the volumetric mass-transfer coefficient that describes the efficiency with which oxygen can be delivered to a bioreactor for a given set of operating conditions, is not new. This note is intended to inform those who are not familiar with the measurement method and/or issues that must be considered when making these measurements. It describes advances in… Read More
Measuring kLa Without Biologicals
Biological-free determinations of kLa provide insights into the mass transfer efficiency of reactors as well as any impact resulting from operating conditions or media additives. Values of kLa are calculated from… Read More
Measuring kLa Using a Biological
Determinations of kLa provide insights into the mass transfer efficiency of reactors as well as any impact resulting from operating conditions or media additives. Values of kLa in systems with active biologicals can be calculated from… Read More
Biophysical Characterization of a Cube-Shaped Bioreactor: Mixing and CO2 Stripping Studies
Since the recent shifts towards use of single-use bioreactor (SUB) technology in cell culture processes, fundamental bioreactor design changes have been introduced. These changes concern both the SUB shape and its agitation method. The effects of these newly-introduced parameters on hydrodynamics properties, such as mixing and gas exchanges, are not well understood at this time. This work has focused on the mixing and carbon dioxide (CO2) stripping capabilities in a cube shaped SUB equipped with… Read More
Bioprocess Bags Integrally Equipped with Oxygen or pH Sensors
Single-use bioprocess bags were equipped with optical sensors for O2 or pH. These sensors enable repeat, real-time, minimally invasive in situ measurements. The sensors are incorporated into the bioprocess bags by way of modified LDPE bag ports specifically designed for this purpose. Unlike traditional bag ports… Read More